Felipe da Veiga Leprevost

Bioinformatician
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With a BS in Biological Sciences and in Systems Analysis I have been working with molecular biology since 2005. Now during my PhD in bioinformatics I'm specializing in computational proteomics, developping methods and techniques for shotgun proteomics.

Who is Felipe Leprevost?

Felipe currently holds a double major, the first one in Biological Sciences where he specialized in Molecular and Cellular Biology and Microparasitology. His second major was in Systems Analysis and Development with emphasis on distributed systems. Holds an MA in Cell and Molecular Biology by Fiocruz - RJ since 2009 where he worked in a molecular biology laboratory characterizing proteins with unknown function.
Currently conducts a PhD in bioinformatics and computational biology at the Instituto Carlos Chagas - ICC / Fiocruz - PR where he works with computational proteomics developing methods and tools for shotgun proteomics.
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Biological Skills

During my scientific initiation and my master's program I dealt with different wet-lab techniques in molecular biology including DNA sequencing by Sanger, DNA and protein gel electrophoresis, cloning (traditional and Gateway system) and protein expression. I also have experience in bacterial and parasite culture and cell preparation for different microscopy techniques using light and electrons emission.

I also have knowledge about the latest next-gen sequencing technologies covering sample preparation and basic analysis. Currently I'm working with the latest mass spectrometry technologies available, dealing with basic sample preparation and data analysis.

Molecular Biology

Excellent

Biochemistry

Good

Cell Biology

Excellent

Genetics

Good
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Computational Skills

Analyst's Skills

Unix and Linux

Excellent

Installation, customization and maintenance for the main distros including Debian based, RedHat based and BSD based.

RDBMS and NoSQL

Excellent

PostgreSQL, SQL Server, MySQL, MongoDB

Statistics and A.I.

Good

Basic methods and strategies for data analysis. Basic understanding and implementations of artificial inteligence algorithms.

Project Management

Good

Agile development and system architecture.

Coding Skills

Perl

Excellent

Basic programming, object orientation, web projects, and distributed systems. Moose, DBIx::Class, Catalyst, Dancer, Template Toolkit amog others.

Java

Excellent

Basic programming, web projects and distributed systems. Java Server Pages, Java Server Faces and Hibernate

C#

Good

Basic programming, web projects and distributed systems. LINQ and ALGLIB

HTML5 / CSS

Good

Moderate knowledge in web design.

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Work Experience

Scientific Director

Hexabio Bioinformática

  • Period: March 2013 - Present day
  • Job type: Full-Time
  • Web Site: Hexabio

Hexabio is a brazilian bioinformatics company specialized bioinformatics and Computational Biology.

  • Biological Data Analysis.
  • Training in Bioinformatics techniques and softwares.
  • Software Development.
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Education

Oswaldo Cruz Institute, Fiocruz - PR

PhD in Bioinformatics and Computational Biology

I'm currently working with computational proteomics, more specifically with tools and technique development for shotgun proteomics. For my thesis I'm developing a new algorithm for de novo protein sequencing analysis aiming to improve the current status of this important technique.

Oswaldo Cruz Institute, Fiocruz - RJ

Master's Degree in Molecular & Cell Biology

During my Master's degree program I have worked with the characterization of proteins with unknown function. Our model organism was Trypanosoma cruzi a flagellate parasite with almost 50% of its protein coding genes classifies as hypotetical proteins.

Positivo University, Curitiba

Technological graduation in System Analysis and Development

Emphasis in distributed systems.

Positivo University, Curitiba

Bacharellor in Biological Sciences

Emphasis in Molecular Biology and Microparasitology.

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Scientific Production

Publications

2013

Event Participation

2012

  • Workshop on Bioinformatics and Omics. Havana, Cuba.

  • V Rio de Janeiro Proteomics Network Forum. Rio de Janeiro, Brazil.

  • YAPC::Brasil. São Paulo, Brazil.

  • XXXII Congress of the Brazilian Computation Society. Curitiba, Brazil.

2011

  • XXVII Annual Meeting of the Brazilian Society of Protozoology / XXXVIII Annual Meeting on Basic Reasearch in Chagas Disease. Foz do Iguaçu, Brazil

  • 7th International Conference of the Brazilian Association for Bioinformatics and Computational Biology - X-Meeting. Florianópolis, Brazil.

  • YAPC::Brasil. Rio de Janeiro, Brazil.

2010

  • XXVI Annual Meeting of the Brazilian Society of Protozoology / XXXVII Annual Meeting on Basic Research in Chagas Disease. Àguas de Lindóia, Brazil.

  • II São Paulo Perl Workshop. São Paulo, Brazil.

  • YAPC::Brasil. Rio de Janeiro, Brazil.

Poster / Short Communications in Proceedings of Scientific Meetings

2012

  • Leprevost FV, Lima DB, Carvalho PC. PepExplorer: A Computational Pipeline for Analyzing Proteomes of Non-Canonical Organisms. V Meeting of the Rio de Janeiro Proteomics Forum. Rio de Janeiro, Brazil.

  • Leprevost FV, Lima DB, Carvalho PC. PepExplorer: A Computational Pipeline for Analyzing Proteomes of Non-Canonical Organisms. First Meeting of the Brazilian Proteomics Society. Rio de Janeiro, Brazil.

  • Lima DB, Perez-Riverol Y, Leprevost FV, Nogeuira FCS, Barbosa VC, Domont G, França F, Carvalho PC. Effectively Addressing Massively Complex Proteomic Search Spaces. First Meeting of the Brazilian Proteomics Society. Rio de Janeiro, Brazil.

2011

  • Leprevost FV, Rampazzo RCP, Marchini F, Pavoni DP, Krieger MA, Probst CM. Systemic Analysis of Gene Expression Modulation After Nutritional Stress in Trypanosoma cruzi. XXVII Annual Meeting of the Brazilian Society of Protozoology / XXXVIII Annual Meeting on Basic Research in Chagas Disease. Foz do Iguaçú, Brazil.

  • Vidal N, Preti H, Afornali A, Francisco EL, Leprevost FV, Batista M, Pavoni DP, Marchini F, Probst CM, Krieger MA. Trypanosoma cruzi ORFeome Project v1.0. XXVII Annual Meeting of the Brazilian Society of Protozoology / XXXVIII Annual Meeting on Basic Research in Chagas Disease. Foz do Iguaçú, Brazil.

2010

  • Leprevost FV, Yamamoto F, Rampazzo RCP, Marchini F, Krieger MA, Probst CM, Pavoni DP. Characterization of a TPPP/p25 Protein in Trypanosoma cruzi. XXVI Annual Meeting of the Brazilian Society of Protozoology / XXXVII Annual Meeting on Basic Research in Chagas Disease. Foz do Iguaçu, Brazil.

2008

  • Yamamoto F, Leprevost FV, Preti H, Umaki ACS, Rampazzo RCP, Probst CM, Pavoni DP, Krieger M. Characterization of genes differentially expressed during Trypanosoma cruzi metacyclogenesis. XXIII Annual Meeting of the Brazilian Society of Protozoology / XXXIV Annual Meeting on Basic Research in Chagas Disease. Águas de Lindóia, Brazil.

  • Leprevost FV, Yamamoto F, Preti H, Umaki ACS, Rampazzo RCP, Dallabona AC, Marchini F, Probst CM, Krieger M, Pavoni DP. Characterization of Genes of Unknown Function With a Higher Expression in Trypanosoma cruzi Metacyclic Trypomastigotes. XXXV Annual Meeting on Basic Research in Chagas Disease / XXIV Annual Meeting of the Brazilian Society of Protozoology. Águas de Lindóia, Brazil.

2007

  • Preti H, Pavoni DP, Leprevost FV, Yamamoto F, Marchini F, Rocha J, Batista M, Probst CM, Goldenberg S, Krieger M. Functional Characterization of Differential Expressed Protein During Tcruzi Metacyclogenesis. Annual Meeting of the Brazilian Society of Protozoology / XXXIV Annual Meeting on Basic Research in Chagas Disease. Caxambu, Brazil.

2006

  • Probst CM, Marchini FK, Pavoni DP, Preti H, Biondo CA, Leprevost FV, Baer F, Batista M, Yamamoto F, Goldenberg S, Krieger M. A Trypanosoma cruzi differentially expressed gene clone collection. XXII Annual Meeting of the Brazilian Society of Protozoology / XXXIII Annual Meeting on Basic Research in Chagas Disease. Caxambú, Brazil.

Oral Presentations in Meetings

2012

  • Big Bio Data. YAPC::BRasil 2012. São Paulo. Brazil.

  • PepExplorer: Organizing and Filtering of de novo Results. Workshop on Bioinformatics and Omics. Havana, Cuba.

Lectures

2013

Event Organization

2013

  • YAPC::Brasil 2013 (in preparation).

2012

  • YAPC::Brasil 2012.

Short Courses

2012

  • Hadoop: basic concpets and practice. Brasilian Computation Society. Curitiba, Brazil.

  • Free Software and Intelectual Propriety. Brasilian Computation Society. Curitiba, Brazil.

2011

  • Bioinformatics Practical Course. Brazilian Association for Bioinformatics and Computational Biology. Florianópolis, Brazil.

  • Free Software Development. Brazilian Association for Bioinformatics and Computational Biology. Florianópolis, Brazil.

2009

  • International Systems Biology Course. Fiocruz. Rio de Janeiro, Brazil.

2007

  • II Workshop on Structural Biology. Campinas, Brazil.

2006

  • Introduction to Electron Mycroscopy and Nanotechnology. Brazilian Society for the Progress of Science. Florianópolis, Brazil.

2005

  • Basic Topics in Bioinformatics. Parana Molecular Biology Institute. Curitiba, Brazil.

  • Molecular Genetics. Brazilian Society of Clinical Genetics. Curitiba, Brazil.

  • Advanced Topics in Parasitology. Brazilian Society of Parasitology. Porto Alegre, Brazil.

Non-Scientific Publications

2013

2012

Softwares

2013

2012

  • Leprevost FV, Lima DB, Carvalho PC. PepExplorer, de novo sequencing analysis

  • Leprevost FV. Bio::Tools::Alignment::Overview, a birds-eye viewer for large multiple alignments.

  • Leprevost FV. CobWeb, a descentralized distributed system based on complex networks.

  • Leprevost FV. TrypViewer, an ultrafast desktop application for Trypanosomatid genomical retrieval.

  • Leprevost FV, Júnior ACP, Emiliano J. Selenide, A distributed system for remote media management.

2011

  • Leprevost FV, Probst CM, TrypanosOmics, a web based system for data visualization for the T.cruzi Orfeome project (not published)

  • Leprevost FV. Liquid, a laboratory stock control focused on next-gen plataforms.

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